(e) Multiplex PCR of Hf2 (Petunia amplicon 408 bp, Calibrachoa amplicon 423 bp) and Hsp70 (Petunia amplicon 569 bp, Calibrachoa amplicon 515 bp) in five different varieties of Supercal (1 – Supercal Cherry, 2 – Supercal Neon rose, 3 – Supercal Pink, 4 – Supercal Purple, 5 – Supercal Terracotta), P24 and C32. ![]() (c, d) Multiplex PCR of Adh1 (Petunia amplicon 223 bp, Calibrachoa amplicon 151 bp) and Hf1 (Petunia amplicon 284 bp, Calibrachoa amplicon 431 bp) in 10 different varieties of Petunia (P) and 12 different varieties of Calibrachoa (C). (a, b) Multiplex PCR of Hf2 (Petunia amplicon 408 bp, Calibrachoa amplicon 423 bp) and Hsp70 (Petunia amplicon 569 bp, Calibrachoa amplicon 515 bp) in 10 different varieties of Petunia (P) and 12 different varieties of Calibrachoa (C). Similar methods can be used for molecular characterization of other new hybrids made between closely related species or cultivars. that the other parent, Petunia, was diploid. Flow cytometry demonstrated that the hybrids were triploid, and determination of chromosome numbers showed that two of the chromosome sets were from Calibrachoa, i.e. PCR primers specific for Petunia and Calabrachoa were made from the determined sequences and tested on a range of presumed hybrids using multiplex PCR, which confirmed that the hybrids were derived from these two species. ![]() For investigation of interspecific hybrids between Petunia and Calibrachoa we used four different introns with flanking exon areas, each from a different gene and with a different sequence for Petunia and Calibrachoa, as genus-specific markers for chromosomes in interspecific hybrids. Commercially, it is useful to combine desirable characteristics of Calibrachoa, including the large diversity of flower colors, with the pH tolerance and vigorous growth of Petunia. Petunia and Calibrachoa are ornamental plants of worldwide economic importance.
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